총 67페이지
4페이지 본문시작
목
차
I. 연구목적 및 필요성·····················································································································
1. 연구목적··························································································································
가. 긴병꽃풀_Glechoma grandis·····························································································1
나. 선행연구 결과·······················································································································
다. 관련 연구현황 및 유사연구 사례························································································4
2. 연구 필요성 및 기대효과···········································································································5
가. 연구 필요성························································································································
나. 미백 관련 국내외 동향·········································································································7
다. 기대효과·························································································································�
II. 재료 및 방법·······················································································································�
1. 실험 재료 및 시료의 추출·······································································································12
2. 실험 방법·························································································································�
가. 생리활성 측정 방법·············································································································13
1) 총 폴리페놀 함량 측정·····································································································13
2) Electron donating ability 측정···················································································13
+
3) ABTS
radical 소거능 측정···························································································13
4) SOD-유사활성 측정··········································································································13
5) Tyrosinase 저해활성 측정·····························································································14
6) Elastase 저해활성 측정···································································································14
나. 세포 생존율 측정·················································································································15
1) 세포주 및 세포 배양········································································································15
2) MTT assay에 의한 세포 생존율 측정·········································································15
다. Western Blot을 통한 단백질 발현 측정········································································15
라. Reverse transcription-PCR을 통한 mRNA 발현 측정···········································16
1) RNA 추출 및 cDNA 합성····························································································16
2) Reverse transcription-PCR·······················································································16
마. 진탕 플라스크법을 통한 항균력 측정·············································································18
1) 사용 균주·························································································································�
2) 진탕 플라스크법(Shake Flask Test)·········································································18
III. 실험 결과························································································································�
1. 생리활성 검증 결과··················································································································19
차
I. 연구목적 및 필요성·····················································································································
1. 연구목적··························································································································
가. 긴병꽃풀_Glechoma grandis·····························································································1
나. 선행연구 결과·······················································································································
다. 관련 연구현황 및 유사연구 사례························································································4
2. 연구 필요성 및 기대효과···········································································································5
가. 연구 필요성························································································································
나. 미백 관련 국내외 동향·········································································································7
다. 기대효과·························································································································�
II. 재료 및 방법·······················································································································�
1. 실험 재료 및 시료의 추출·······································································································12
2. 실험 방법·························································································································�
가. 생리활성 측정 방법·············································································································13
1) 총 폴리페놀 함량 측정·····································································································13
2) Electron donating ability 측정···················································································13
+
3) ABTS
radical 소거능 측정···························································································13
4) SOD-유사활성 측정··········································································································13
5) Tyrosinase 저해활성 측정·····························································································14
6) Elastase 저해활성 측정···································································································14
나. 세포 생존율 측정·················································································································15
1) 세포주 및 세포 배양········································································································15
2) MTT assay에 의한 세포 생존율 측정·········································································15
다. Western Blot을 통한 단백질 발현 측정········································································15
라. Reverse transcription-PCR을 통한 mRNA 발현 측정···········································16
1) RNA 추출 및 cDNA 합성····························································································16
2) Reverse transcription-PCR·······················································································16
마. 진탕 플라스크법을 통한 항균력 측정·············································································18
1) 사용 균주·························································································································�
2) 진탕 플라스크법(Shake Flask Test)·········································································18
III. 실험 결과························································································································�
1. 생리활성 검증 결과··················································································································19
4페이지 본문끝
메뉴
현재 포커스의 아래내용들은 동일한 컨텐츠를 가지고 페이지넘김 효과및 시각적 효과를 제공하는 페이지이므로 스크린리더 사용자는 여기까지만 낭독하시고 위의 페이지이동 링크를 사용하여 다음페이지로 이동하시기 바랍니다.